Azalea and Berry Vine

The biological parents of Anthea (Annie). Their whereabouts are unknow, their reason for leaving Anthea is unknown and whether they’re dead or alive is also unknown

Fluttershy

Anthea’s adoptive mother. Fluttershy adopted Annie when she was around 4 or 5 years old from the Ponyville orphanage.

Button Mash and Sweetie Belle

The parents of Pixel Bit. They live in Ponyville but travel a lot.

Rarity and Spike

The parents of Crystal Clairty (Claire) and Turquoise Blitz (T). After they married, they lived in Canterlot until for Rarity’s job. They moved back to Ponyville when T was 5 years old.

Celestia and Discord

The parents of Illusion.

Luna and Supernova

The parents of Nidra. Supernova is one of. Luna’s nightguards.

Flash Sentry and Twilight Sparkle

The parents of Starburst (Star) and Nightlight (Nighty). Over the years, things became very busy in Equestria and there were plenty threats along the way. As result, Twilight and Flash ended up spending a lot of time in Canterlot rather than in Ponyville.

Shining Armor and Cadence

The parents of Valiant Heart (Val/Lance). They rule the Crystal Empire.

Pinkie Pie and Pokey Pierce

The parents of Cotton Candy, Cloudy Skies (Sky) and Sugar Rush. They live in Ponyville.

Scootaloo and Rumble

The parents of Echo. They live in Ponyville.

Thunderlane and Cloudchaser

The parents of Whirlwind. They live in Cloudsdale.

Rainbow Dash and Soarin

The parents of Prism Bolt (Priz) and Icy Storm. Rainbow used to live in Ponyville but moved to Cloudsdale with Soarin after marriage.

Cheerilee and Big Macintosh

The parents of Red June. They live in Ponyville but not Sweet Apple Acres. Big. Mac built a house nearby the farm for themselves for privacy and so Sweet Apple Acres wouldn’t be too crowded.

Applejack and Caramel

The parents of Golden Delicious (Del). They live on Sweet Apple Acres in Ponyville.

Apple Bloom and Pipsqueak

The parents of Api Apple. They also live off the farm. However, Apple Bloom still helps out on the farm and when. Pip goes on his travels, she stays at the farm along with Api.

Fleur de Lis and Fancy Pants

The parents of Crme de la Crme. They live in Canterlot.

Next Gen From Left to Right (general bios coming soon):

Anthea(Annie)

Pixel Bit (Pixie)

Turquoise Blitz (T)

Crystal Clarity (Claire)

Illusion

Nidra

Starburst (Star)

Night Light (Nighty)

Valiant Heart (Val/Lance)

Cotton Candy (CeeCee)

Cloudy Skies (Sky)

Sugar Rush

Echo

Whirlwind

Prism Bolt (Priz)

Icy Storm

Red June

Golden Delicious (Del)

Api Apple

Crme de la Crme