Dragon Lord Ember and Starlight Glimmer are both lesbian. They are no longer a couple (Starlight is married to Sunset Shimmer now), but when they were still together, they were inseparable. After they broke up, Ember swore never to love again. Her love for Starlight is timeless. Starlight still regrets breaking up with her, which she only did because she was worried she and Ember would not be functional long-term or long distance. Ember was, and always would be the Dragon Lord, and there was nothing Starlight could ever do to change that. She feared one day Ember would take priority of the Dragonlands and choose her people over her, so she left Ember before Ember could even think about leaving her. Not a day goes by where Ember doesn't think of her.

They have no children together, but Ember always wanted to start a family with her. If you're curious, it is possible for a dragon and pony to have a kid, although it would be a hybrid and infertile.

Clear Sky and Rainbow Dash are my otp, and I'll never stop gushing about them. They were included in last year's pride upload- they're both bisexual. Clear Sky has no gender preference, while Dash has a preference for mares.

They have 4 children together: Thunderbird (bisexual), Greywind (asexual lesbian), Astra (polyamorous, gender fluid, and pansexual) and Wind Sprint (asexual, greyromantic, biromantic). Technically, Wind Sprint is Rainbow Dash's adopted daughter, since she is fathered by Clear Sky's previous boyfriend, but Dash considers Sprint her own.

Twilight Sparkle is bisexual, as are all of the Sparkle children (Shining Armor and Spike). Tempest Shadow is lesbian. After Tempest is given a second chance, Twilight requests her as her personal royal guard. Tempest swears fealty to her, and vows to shield her back and give her life for Twilight's if it ever comes to that. She does not take this vow lightly. Throughout her time as Twilight's guard, they both start to fall in love with each other. Twilight's not so subtle about it.

They have 1 kid together: Cassiopeia, former heir to the throne of magic, currently exiled from Equestria. Cassie is a lesbian, she just doesn't know it yet.

Also, because so many people care about this sort of thing: in my headcanon, same-sex couples can procreate. They don't need a sex change spell, they don't need magical help of any kind, they don't need a sperm or egg donor, none of that. They just can. In a world of mostly girl ponies I really don't think it's that much of a stretch to say it's just physically possible for them.

You guys can safely assume that all of my characters are LGBT unless I say otherwise, and the same goes for my headcanons on canon characters.